To compare the efficacy of cardiac preservation, we examined purine metabolites during 24 hours of cold storage(0℃) of the Korean mongrel dog hearts after using three different types of cardioplegic solutions. The hypothermic arrest with total
cardiopulmonary bypass method was employed in St Thomas solution(STS) and blood cardioplegic solution(BCPS) preservation cases.
Specimens were analyzed for levels of adenine nucleotides and their precursors by high performance liquid chromatography.
The ATP content in the UW(University of Wisconsin) solution group tends to be higher than that of the combined hypothermic arrest group(STS and BCPS groups) after 2, 4, 8, and 12 hours of preservation respectively, but there were no significant
differences between STS and BCPS group.
The ADP conte4nts in the UWS and BCPS groups were higher than that of the STS group at 4, 8, 12, and 24 hours, but the difference was not statistically significant between UWS and BCPS groups.
The AMP contents did not change significantly in the three groups. The adenosine, inosine, and hypoxanthine concentrations increased progressively, but the level of xanthine was very low in the three groups.
This study strongly suggests that UWS did not provide satisfactory long-term preservation of heart, but was superior than STS and BCPS group with hypothermic arrest method.
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